in the present study, genotyping of six short tandem repeat (str) loci including csf1po, d16s539, f13a01, f13b, lpl and hprtb was performed on genomic dna from 127 unrelated individuals from the iranian province of isfahan. the results indicated that the allele and genotype distributions were in accordance with hardy-weinberg expectations. the observed heterozygosity (ho), expected heterozygosi...

MOTIVATION Long expansions of short tandem repeats (STRs), i.e. DNA repeats of 2-6 nt, are associated with some genetic diseases. Cost-efficient high-throughput sequencing can quickly produce billions of short reads that would be useful for uncovering disease-associated STRs. However, enumerating STRs in short reads remains largely unexplored because of the difficulty in elucidating STRs much l...

We identified and characterized 14 novel short-tandem-repeats (STRs) on the Y chromosome and typed them in two samples, a globally diverse panel of 73 cell lines, and 148 individuals from a European-American population. These Y-STRs include eight tetranucleotide repeats (DYS449, DYS453, DYS454, DYS455, DYS456, DYS458, DYS459, and DYS464), five pentanucleotide repeats (DYS446, DYS447, DYS450, DY...

objective: classic cell culture and karyotyping is routinely used for prenatal detection of different chromosomal abnormalities. molecular cytogenetic techniques have also recently been developed and used for this purpose. quantitative florescence pcr using short tandem repeat (str) markers has more potential for high throughput diagnosis. marker heterozygosity in short tandem repeats (str) is ...

Although the utility of short tandem repeats on the Y-chromosome (Y-STRs) has long been recognized and leveraged in forensics, genealogy and paternity testing, the bulk of these applications have relied on only a few dozen loci identified as having remarkably high mutation rates. Recent efforts have expanded the set of Y-STRs with known mutation rates to two hundred markers, but the limited thr...

BACKGROUND Polymorphic Y chromosome short tandem repeats (STRs) have been widely used in population genetic and evolutionary studies. Compared to di-, tri-, and tetranucleotide repeats, STRs with longer repeat units occur more rarely and are far less commonly used. PRINCIPAL FINDINGS In order to study the evolutionary dynamics of STRs according to repeat unit size, we analysed variation at 24...

objective(s): short tandem repeat (str) loci are the most informative dna genetic markers for attempting to individualize biological material for application in paternity and forensic cases. materials and methods: blood samples were collected and the total genomic dna was extracted. the dna samples were used for genotyping vwa and tpox str loci using pcr and polyacrylamide gel electrophoresis. ...

Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and found that a multiplexed PCR assay using primers based upon these STRs accurat...

OBJECTIVES Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) are a useful biological resource, however, genomic variations can happen during the generation and immortalization processes of LCLs. The purpose of this study was to identify genomic variations in LCL DNA compared with matched blood DNA using short tandem repeats (STRs) analysis. METHODS We analyzed 15 STRs with...

Genome sequencing of the protistan parasite Entamoeba histolytica HM-1:IMSS revealed that almost all the tRNA genes are organized into tandem arrays that make up over 10% of the genome. The 25 distinct array units contain up to 5 tRNA genes each and some also encode the 5S RNA. Between adjacent genes in array units are complex short tandem repeats (STRs) resembling microsatellites. To investiga...